mouse monoclonal primary antibodies for osteocalcin  (R&D Systems)

Journal: Journal of Materials Science. Materials in Medicine

Article Title: Cissus quadrangularis L extract-loaded tricalcium phosphate reinforced natural polymer composite for guided bone regeneration

doi: 10.1007/s10856-023-06739-x

Figure Lengend Snippet: a (A) Quantitative MTT cell proliferation assay and RT-PCR cell differentiation analysis on the specific markers including (B) RUNx2, (C) OCN, (D) VEGF after treating with β-TCP, β-TCP/Gel-Pec, and β-TCP/Gel-Pec/CQ composites for 1, 3, 7 and 14 days of treatment. *Comparison of the indicated group with control cells within the same set. * p < 0.05. # Comparison of the indicated group with mHAP ceramic within the same set. # p < 0.05. **Comparison of the indicated group with β-TCP/Gel-Pec/CQ composite within the same set. ** p < 0.05. b PCR images of RUNx2 and OCN genes

Article Snippet: The membrane was then treated with mouse monoclonal IgG primary antibodies (Osteocalcin, Cat # SC-73464; Runx2, Cat #SAB1412665 from Sigma–Aldrich, India) at 4 °C overnight after being blocked for 1 h at room temperature with 5% non-fat milk in PBS.

Techniques: MTT Cell Proliferation, Reverse Transcription Polymerase Chain Reaction, Cell Differentiation

Journal: Bone reports

Article Title: Role of cartilage and bone matrix regulation in early equine osteochondrosis.

doi: 10.1016/j.bonr.2023.101653

Figure Lengend Snippet: Fig. 5. Photomicrographs depicting protein expression for osteocalcin following immunohistochemical localization. A) Osteochondral sample of foal with confirmed OC showing moderate to strong osteocalcin expression in chondrocytes along the osteochondral junction and in within the deeper cartilage layers. B) Negative control for (A) following substitution of mouse monoclonal osteocalcin antibody with nonimmune serum. C) Osteochondral samples of normal foal showing similar results (bar = 100 μm).

Article Snippet: Immunohistochemistry (IHC) was performed on paraffin-embedded osteochondral sections using mouse monoclonal primary antibodies for Osteocalcin (cat#MAB1419, R&D Systems, Inc., Minneapolis, MN) and Collagen type IIB (clone M2139, Thermo Fisher Scientific, Waltham, MA) and rabbit polyclonal primary antibodies for Lubricin (ab28484, Abcam, Cambridge, UK) and SOX9 (PA5-23383, Thermo Fisher Scientific, Waltham, MA).

Techniques: Expressing, Immunohistochemical staining, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Biological Response to Macroporous Chitosan-Agarose Bone Scaffolds Comprising Mg- and Zn-Doped Nano-Hydroxyapatite

doi: 10.3390/ijms20153835

Figure Lengend Snippet: CLSM images presenting immunofluorescent (IF) staining of type I collagen and osteocalcin in the extracellular matrix (ECM) of MSCs cultured in PS wells in the presence of scaffolds extracts (control medium, cells maintained in osteogenic medium); collagen, red fluorescence; osteocalcin, green fluorescence; and nuclei, blue fluorescence, magnified 400×; scale bar = 50 µm.

Article Snippet: Then, the samples were incubated overnight at 4 °C with primary goat anticollagen I (Col1a1/Col1a2) polyclonal antibody (Abnova, Taoyuan City, Taiwan) and primary mouse anti-osteocalcin monoclonal antibody (Abcam, Cambridge, UK) prepared at a concentration of 10 μg/mL.

Techniques: Staining, Cell Culture, Control, Fluorescence